13th Congress of the Hungarian Neuroscience Society
20th - 22th Jan, 2011
Budapest, Hungary
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NEU-06-SEN SensoStress
The classical methods used for investigation of thermonociception measure the latency of nocifensive reactions to noxious heat stimuli of constant suprathreshold intensity. They include the hot plate, tail flick and the plantar (Hargreaves) tests. These methods do not allow comparison of results obtained in behavioral and electrophysiological studies. By these the methods the threshold temperature needed for channel or unit activation is measured and behavioral latency has no electrophysiological counterpart. Experimetria developed an increasing-temperature water bath which allows determination of the noxious heat threshold temperature of conscious rats. It’s also developed a hyperalgesia model based on mild heat injury and assessed the antihyperalgesic effects of various analgetics. The noxious heat threshold was defined as the temperature at which the animal withdrew the hind paw from the water bath, which was heated up from a predetermined temperature (30°C) at a rate of 24 °C/min. Heat injury was induced by immersing one of the hindpaws in a 51 °C hot water bath for 20 seconds under brief aether anaesthesia. Following recovery from anaesthesia heat threshold determinations were repeated and drugs were administered 20 min later either i.p. or intraplantarly. DETAILED INFORMATION ( PDF) European Journal of Pharmacology article (PDF)
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