13th Congress of the Hungarian Neuroscience Society
20th - 22th Jan, 2011
Budapest, Hungary
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NEU-03-BSS System for Recording Evoked Potentials in brain slices
BSS-01 tissue bath (Haas type) is designed to perform electrical biopotential measurements extra-, or intracellularly on isolated brain slices. The bath is mounted on a base plate, which is equipped with holder for AM-04 manipulator. It holds electrodes for electrical stimulation. The lower plexi glass chamber is filled with distilled water and bubbled with carbogen. The upper plexi glass chamber supports the slice. Temperature is kept constant by a regulated heater placed in the lower plexi glass chamber. A small upper lid covers the upper plexi glass chamber. This lid directs the flow of warmed, moisturized and carbogen rich air onto the surface of the slice to avoid drying and supplying it with oxygen. The bottom of the slice is also superfused with warmed ACSF saturated with carbogen. The lid covers only a small part of the upper chamber, so the slice is easily accessible for electrophysiological manipulations. A widely used brain slice preparation is the transverse hippocampal slice. It allows recording large population spikes from pyramidal cell layer of CAI region when Schaffer collateral axons are stimulated. It is an excellent method for studying both extracellular and intracellular signals recorded with specially designed combined intra- and extracellular amplifiers. Dimensions width: 200 mm, length: 250 mm, height: 300 mm (with base plate), weight: 2kg (with base plate) |
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